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fluorescence microscopy

Charting a New Course Through the Injured Brain

Rashmi Shivni | Jan 15, 2024 | 4 min read

A state-of-the-art technique helps scientists map out tissue at the single cell level after a demyelinating brain injury.

An image of neurons, astrocytes, and other glial cells acquired using fluorescence microscopy.

Troubleshooting Fluorescence Microscopy Experiments

The Scientist and Evident | Nov 10, 2023 | 1 min read

Delve into the tactics used by scientists to overcome fluorescence microscopy’s greatest obstacles.

Illuminating Specimens Through Live Cell Imaging

Charlene Lancaster, PhD | 8 min read

Live cell imaging is a powerful microscopy technique employed by scientists to monitor molecular processes and cellular behavior in real time.

Illustration of newly discovered mechanism allowing kinesin to “walk” down a microtubule. A green kinesin molecule with an attached yellow fluorophore is shown passing through a blue laser as it rotates step by step along a red and purple microtubule, fueled by blue ATP molecules that are hydrolyzed into orange ADP and phosphate groups.

High-Resolution Microscope Watches Proteins Strut Their Stuff

Holly Barker, PhD | Mar 31, 2023 | 3 min read

Modification on a high-resolution fluorescent microscopy technique allow researchers to track the precise movements of motor proteins.

To flag neurons that have experienced genotoxic stress, researchers developed an in vivo sensor using an adeno-associated viral vector, called PRISM. Because a cell’s DNA damage response (DDR)—which activates in response to stressors such as environmental toxins or the buildup of misfolded proteins—also responds to invading pathogens, PRISM has an easier time transfecting cells whose damage response mechanisms are preoccupied with existing DNA damage. Once inside, the virus hijacks the neuron’s DNA replication machinery, which reverts an engineered frameshift mutation in the virus and thereby prompts the production of a fluorescent protein that can be observed via microscopy.

Infographic: DNA Damage Viewed with Unprecedented Clarity

Amanda Heidt | Aug 15, 2022 | 2 min read

A new genetic sensor called PRISM makes use of a host cell’s DNA replication machinery to trigger fluorescence in neurons with damaged DNA.

Dream Big and Achieve Real-Time Single Cell Imaging Without Camera Limitations

Sort What You See

BD Biosciences | 1 min read

Camera-free imaging unlocks new cell sorting applications.

DNA Damage Viewed with Unprecedented Clarity

Amanda Heidt | Aug 15, 2022 | 3 min read

A new tool called PRISM draws on virus-host interactions and a DNA repair pathway to help researchers visualize how cellular stress may contribute to neurodegenerative disease.

Microscopic image of nerves in the eye, a pathogen, and t cells

Science Snapshot: Eye Immunity

Lisa Winter | May 26, 2022 | 1 min read

Researchers find that tissue-resident memory T cells in the corneas of mice engender a lasting immune response.

Peering into the Cell

The Scientist | 1 min read

Researchers visualize the beautiful inner world of cells!

A scanning electron micrograph of a coculture of E. coli and Acinetobacter baylyi. Nanotubes can be seen extending from the E. coli.

What’s the Deal with Bacterial Nanotubes?

Sruthi S. Balakrishnan | Jun 1, 2021 | 10+ min read

Several labs have reported the formation of bacterial nanotubes under different, often contrasting conditions. What are these structures and why are they so hard to reproduce?